Comparison of three commercial sparse-matrix crystallization screens

Crystallization Screens Crystallization Optimization Cryo Screens Phasing

Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility.

Nuclear magnetic resonance (NMR) and crystallographic techniques require large quantities of pure, soluble protein. However, numerous target proteins exhibit limited solubility resulting in low recovery and reduced concentrations following purification. Molecular biological techniques have been developed to assist in this area including the use of a fusion protein attached to the N or C terminu...

Crystallization screens: compatibility with the lipidic cubic phase for in meso crystallization of membrane proteins.

The in meso method for growing crystals of membrane proteins uses a spontaneously forming lipidic cubic mesophase. The detergent-solubilized protein is dispersed with lipid, typically monoolein, and in so doing the cubic phase self-assembles. A precipitant is added to trigger crystal nucleation and growth. The commercial screen solution series are convenient for use in crystallization trials. T...

Comparison of Eigenvalue Solvers for Large Sparse Matrix Pencils

Efficient and accurate computation of eigenvalues and eigenvectors is of fundamental importance in the accelerator physics community. Moreover, the eigensystem analysis is generally used for the identifications of many physical phenomena connected to vibrations. Therefore, various types of algorithms such that Arnoldi, Lanczos, KrylovSchur, Jacobi-Davidson etc. were implemented to solve the eig...

An Empirical Comparison of Three Commercial Information Visualization Systems

An empirical comparison of three commercial information visualization systems on three different databases is presented. The systems use different paradigms for visualizing data. Tasks were selected to be "ecologically relevant", i.e. meaningful and interesting in the respective domains. Users of one system turned out to solve problems significantly faster than users of the other two, while use...